DNA

Part:BBa_K2100026

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pENTR VgEcR VgEcR is part of the ecdysone inducible expression system. The synthetic receptor fusion VgEcR forms a complex with RXR which binds as a heterodimer to the E/GRE recognition sequences on the EGSH promoter, transcribing the gene of choice downstream of the promoter. This system can be induced with the small molecule PonA, and is otherwise tightly controlled by corepressors.[1]

[1]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2091
    Illegal PstI site found at 2272
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1585
    Illegal BamHI site found at 486
    Illegal BamHI site found at 2962
    Illegal BamHI site found at 3115
    Illegal BamHI site found at 3229
    Illegal XhoI site found at 1578
    Illegal XhoI site found at 1659
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
    Illegal NgoMIV site found at 1707
    Illegal NgoMIV site found at 2054
    Illegal NgoMIV site found at 3527
    Illegal AgeI site found at 2815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3000
    Illegal SapI.rc site found at 389
    Illegal SapI.rc site found at 521


[edit]
Categories
//awards/part_collection/2016
Parameters
None